Project description

Tuesday - 22/12/2015 02:52

 The laboratory is leaded by Pr. Pascal Gantet (UM2/IRD) and Pr. Do Nang Vinh (AGI).It involves in Vietnam French researchers from UM2, IRD and CNRS (expatriates, long term missions) and Vietnamese researchers from AGI, USTH and from different institutes of VAAS such as Plant Protection Research Institute (PPRI), and Plant Resource Center (PRC). Our core and emerging activities involve researchers or join projects with the main UMR working on plant functional genomics in Montpellier mainly DIADE (UM2/IRD) and RPB (IRD/UM2/CIRAD) but also AGAP (CIRAD, INRA, SupAgro), BPMP (CNRS, INRA, UM2, SupAgro) and BGPI (INRA, CIRAD, SupAgro).

The research activities conducted in LMI RICE focus on rice, the most important crop in Vietnam and in two main axes: study of development in regards to abiotic stress resistance and yield, and plant resistance to biotic stresses, by combining genetic diversity studies, association genetics, functional genomics and biotechnology approaches. The main achievements of these two last years and immediate perspectives are:

-The definition of a core collection of 188 traditional rice varieties collected from North to South Vietnam and adapted to different water culture regimes. This collections have been genotyped with 30 000 markers by DaRT sequencing that revealed a representation of all main sub-group of rice: indica, japonica, ausboro, sadri-basmati. In addition a full rough (1X) sequencing of the genome of each variety is planed (IRIGIN project). The root system and panicle of these varieties have been phenotyped and we plan a phenotyping of the collection for tolerance to drought stress, salt stress, water conductivity capacity, and the capacity of plant to maintain yield in drought conditions. We expect by genotype/phenotype association studies to identify new genes and alleles involved in root development, abiotic stress resistance and yield and yield maintenance in stress condition. The functional validation of the genes and alleles will be done in LMIRICE and the transfer of valuable genes in most popular varieties by molecular assisted selection will be developed in partnership with CIRAD and the International Rice Recarch Institute in Philippine (IRRI) with which we are already interacting for genotyping and phenotyping purposes. In addition we are validating the function of different candidate genes controlling root development previously identified by transcriptome and the intracellular dynamics ofaquaporins in root in normal or osmotic stress condition.

 - Collect and investigate the genetic diversity studies of the main rice pathogens in SE Asian region (Virus, root knot nematodes, Xanthomonas). These diversity studies aims to better understand the distribution and the mechanisms of evolution and emergence of the main pathogens affecting rice culture. It is an indispensable preliminary step to screen rice for natural resistance, to better understand the key molecular actors involved in the plant/pathogeninteractions and to design biotechnological strategies to create large spectrum and durable resistances in rice. This work has been initiated for two main rice viruses for which diversity have been investigated and studied in Vietnam. Biological activities of the main proteins encoded by virus genomes are studied, and RNA interference based strategies targeting conserved virus sequences are in course of evaluation in rice to create resistance. These activities are included in a regional network GDRI “Infectious diseases, biodiversity and Health risk in SE Asia”. USTH have been included in the second edition of this program. Beside a work aiming to determine some major components of the molecular crosstalk in nematode/rice interaction a first survey and nematode diversity study in Vietnam has been achieved. It will be soon extended to neighbor countries with the support of the BIOASIA project involving different researchers from different SEAsia countries. Taking together these approaches will allow to define strategies to create resistances in rice against pathogens. Similar work on Xanthomonas has been initiated. End of 2012 a workshop organized in PPRI lead to the creation of the “Surveillance Network on Rice health in South East asia” network SUNRICE that federates researchers from LMI RICE and Thailand, Philippines, Lao and Cambodgia.

In Addition to these networks, the LMI RICE activities are included in the regional Asia network from IRD “PPR SELTAR” and develop in close relation with IRRI in the scope of the GRiSP megaprogam.

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